qPCR Instruments and Reagents

Generate results you can trust.

Amplify your success in real-time PCR applications with the LightCycler® Instruments. Choose the system that best suits your needs, with flexible sample volumes and throughputs ranging from 32 to 384 samples per run.

All of our instruments provide outstanding temperature uniformity and homogeneity, as well as unbiased data capture, enabling you to quickly generate results.

See our instruments below:

Intuitive for all levels of experience

The LightCycler® 96 System is a mid-throughput qPCR solution that enable research breakthrough. Innovative design of optics and thermal block provide an ideal combination of accuracy, temperature homogeneity, and reproducibility for unbiased result research requires.

Comprehensive programming options, a large touchscreen and intuitive analysis software make it even easy to obtain publication-ready results.

The LightCycler® 96 System

Versatile for experienced users

The LightCycler® 480 System is a high-performance, flexible throughput qPCR platform (interchangeable 96- or 384-well blocks) that provides a plate-based, highly adaptable and versatile real-time PCR system for gene expression analysis, SNP genotyping, and mutation scanning via high resolution melting (HRM). The modern instrument design, outstanding technical features, and comprehensive software make the LightCycler® 480 System your platform of choice for high speed and accuracy in all current real-time PCR applications.

 

The LightCycler® 480 System

Rely on Roche proprietary PCR enzymes and formulations to consistently deliver exceptional sensitivity* and specificity for your real-time PCR assays.

Choose the reagent that is best for your experiments on LightCycler® instruments or non-Roche qPCR instruments.

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* For example, based on the results of head-to-head tests of the LightCycler® EvoScript RNA SYBR® Green I Master versus the SuperScript® III Platinum® SYBR® Green One-Step qRT-PCR Kit and the LightCycler® EvoScript RNA Probes Master versus SuperScript® III Platinum® One-Step qRT-PCR Kit. Tests were run with various sample materials using the optimal conditions specified in the instructions for use for all products. For the LightCycler® Multiplex DNA Master, based on the results of the efficiency and linearity of singlesplex and 4-plex of four different assays on the LightCycler® 96 System, and 1:4 dilutions and triplicate multi-operator assays in singleplex and duplex on the LightCycler® 480 System.

Choose from a variety of Roche probe-based qPCR assay solutions for your gene expression needs. Opt for universal probes for affordable and easy-to-design assays, or select function-tested single assays and customised panels to skip design and optimisation steps. Rely on Roche qPCR assay solutions to generate reliable and consistent real-time PCR results*, along with complete assay information to facilitate compliance with the MIQE guidelines.

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* For example, based on the results of head-to-head tests of the LightCycler® EvoScript RNA SYBR® Green I Master versus the SuperScript® III Platinum® SYBR® Green One-Step qRT-PCR Kit and the LightCycler® EvoScript RNA Probes Master versus SuperScript® III Platinum® One-Step qRT-PCR Kit. Tests were run with various sample materials using the optimal conditions specified in the instructions for use for all products. For the LightCycler® Multiplex DNA Master, based on the results of the efficiency and linearity of Singleplex and 4-plex of four different assays on the LightCycler® 96 System, and 1:4 dilutions and triplicate multi-operator assays in Singleplex and duplex on the LightCycler® 480 System.

Molecular Lab

cobas® 4800 system
 
 
cobas® 6800 / 8800 systems
 
 
COBAS® AmpliPrep / COBAS® TaqMan® system
 
cobas p 480 instrument
 
 
 
MagNA Pure System Family
 
 
The FLOW Solution